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灵芝体外抑制猴免疫缺陷病毒作用的研究

Study on Effects of Inhibition of Ganoderma lucidum on Simian Immunodeficiency Virus in vitro

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【作者】 余雄涛谢意珍李婷梁耀光蔡勉华彭涛王弋潘鸿辉

【Author】 YU Xiong-tao1,XIE Yi-zhen1,LI Ting2,LIANG Yao-guang1,CAI Mian-hua1, PENG Tao2,WANG Yi2,PAN Hong-hui1(1.Guangdong Institutes of Microbiology,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Yuewei Edible Fungi Technology Co.,Ltd.,Guangzhou 510663,China; 2.Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China)

【机构】 广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室广东粤微食用菌技术有限公司中国科学院广州生物医药与健康研究院

【摘要】 目的:对灵芝孢子水提物(AEGLS)进行体外抗猴免疫缺陷病毒(SIV)活性分析并对其作用机制进行初步研究。方法:300 TCID50的SIV病毒稀释液和AEGLS稀释液同时加入到CEM×174细胞(CEM T淋巴细胞和174 B淋巴细胞杂交细胞)中培养4 d后计数合胞体数量并进行统计,研究AEGLS抗SIV活性。利用不同加药时间点实验、作用阶段分析实验、Western blot对其作用机制进行初步分析。结果:AEGLS具有良好的抗SIV活性,其半数抑制浓度IC50为(466.62±20.21)mg·L-1,并且其细胞毒性较低,半数细胞毒性浓度CC50为(3.13±0.11)g·L-1,选择指数为6.71。抗SIV机制研究显示AEGLS主要作用在SIV病毒感染的早期阶段,与抑制SIV病毒的吸附或者穿透细胞有关,Western blot实验显示AEGLS能降低SIV衣壳蛋白P27(SIV P27)的表达水平。结论:AEGLS具有良好的抗SIV作用,其主要作用在SIV病毒感染的早期阶段,与抑制SIV病毒的吸附或者穿透细胞有关,并且能降低SIV P27衣壳蛋白的表达水平。

【Abstract】 Objective:This paper presents the effects of anti simian immunodeficiency virus(SIV) of the aqueous extract from Ganodorma lucidum spores(AEGLS)by using SIVmac and CEM×174 cells as a model in vitro,and the mechanism were preliminarily studied.Method: The inhibitory effects of AEGLS on SIV were observed when SIV and CEM×174 system were employed as the models,and the mechanism of action were preliminarily studied by using time course assay,effective stage analysis,Western blot.Result: AEGLS had a remarkable anti-SIV activity.The IC50(50% inhibitory concentration) of AEGLS was(466.62±20.21)mg·L-1,and it was low cytotoxicity which its CC50(50% cellular cytotoxicity) was(3.13±0.11)g·L-1 and selection index was 6.71.The results of anti-SIV mechanism showed AEGLS main acted on the early stage of SIV infection,and might contribute to affect the SIV attachment and/or penetration into the cells.The results of Western blot showed AEGLS could decrease SIV P27 capsid protein expression level.Conclusion: AEGLS had a remarkable anti-SIV activity and is main acted on the early stage of SIV infection,and might contribute to affect the SIV attachment and/or penetration into the cells,and could decrease SIV P27 capsid protein expression level.

【基金】 广东省中国科学院全面战略合作项目(2009B091300139);高新技术产业化项目(2010A010500007)
  • 【文献出处】 中国实验方剂学杂志 ,Chinese Journal of Experimental Traditional Medical Formulae , 编辑部邮箱 ,2012年13期
  • 【分类号】R285.5
  • 【网络出版时间】2012-05-04 12:08
  • 【下载频次】156
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